Molecular Genetic Approaches to Detect Meat Adulteration
Many studies worldwide emphasized that adulteration of high-priced meat with cheaper meat, is one of the most common examples of fraudulence prevalent in meat industry without any consideration of economic, religious or health implications. Recent surveys in UK, using ELISA technique, have revealed that some meat sold as Halal contain pork. Numerous analytical methods that rely on protein analysis have been developed for identification of the meat-producing species. However, proteins are known to lose their biological activity following animal’s slaughter while their presence and characteristics depend on cell types, most of them are heat-affected. Thus, for species identification, a DNA, rather than a protein, analysis would be preferable. The proposed work aims at establishing a DNA based techniques to detect meat and meat products adulteration.
Proposed Research Objectives
2- Detect the polymorphism of mt cytochrome b gene and 12SrRNA gene in some species commonly and not commonly used for producing meat and meat products for human consumption
3- Conduct fingerprinting for some species commonly or not commonly used for producing meat and meat products for human
2- Sensitive, accurate and rapid molecular genetic technique for detection meat adulteration kit.