Production of new Biopestecides against housefly by molecular Genetics Techniques

إنتاج مبيد حيوي جديد ضد الذبابة المنزلية بتقنيات البيولوجياالجزيئية


Introduction


(Musca domestica), a common insect of the family Muscidae (order Diptera). About 90 percent of all flies occurring in human habitations are houseflies. Once a major nuisance and hazard to public health in cities, houseflies are still a problem wherever decomposing organic waste and garbage are allowed to accumulate.
The housefly, Musca domestica is a well-known cosmopolitan pest of both farm and home. This species is always found in association with humans or activities of humans. This is the most common species found on hog and poultry farms, horse stables, and ranches. Not only are they a nuisance, but they also can transport disease-causing organisms. Excessive fly populations are obnoxious to farm workers, and when there is nearby human habitations a public health problem is possible. More than 100 pathogens associated with the house fly may cause disease in humans and animals.
Application of Cry Proteins for Pest Control and Plant Protection: B. thuringiensis (Bt) is now the most widely used biologically produced pest control agent.
The use of biological control in fly management is still at a relatively early stage. So that the use of biological control and natural enemies against housefly by genetically modified strain of B. thuriengensis are the main point of this project.

Objectives

This project aims to isolate, identify and characterize different Bacillus thuringiensis from different geographical areas. Also, test the effect of these strains against mortality of housefly insects and select the effective strains for its mortality. We aim to improve the selected strains by molecular genetics techniques i.e., plasmid profile and transfer between the same species or different species to over-express the Cry proteins within selected strains especially the most Cry genes coded by plasmids in Bacillus strains. Transposable genetics has been described with regard to their structural association with Cry genes, so that attempts to transpositions using transposable genetic elements from different bacillus species to the selected strains in order to improve their mortality. DNA fingerprinting using specific Cry genes and restriction enzyme pattern will also be performed.     
In this project we aim at producing recombinant commercially Bt strains with a multiple toxicity effect especially against larvae of houseflies diptran by molecular genetics methods.